Background Hyperspectral Imaging (HSI) captures spectral information that has been shown to characterise tissues. Since the surgeon’s naked eye is sometimes unable to differentiate between tumour and normal brain, HSI could be a solution.Aims We aim to develop an intra-operative HSI system capable of tumour detection and delineation.Methods Using a newly developed HSI system that captures images across the visible and near-infrared electromagnetic spectrum (from 400nm to 1700nm); we obtained hyperspectral images during tumour resection. Before imaging, markers were placed at the location of the tumour and normal brain. Images were then captured with the markers in-situ. Tissue samples were obtained from the location of the markers and sent for tissue diagnosis.